Development of biomarkers of intake and effect from a Mediterranean diet for cardivascular preventing funtions. Metabolomic approach.
GENERAL INFORMATION
Call: Convocatòria d'ajuts per a la realització de projectes de recerca i accions complementàries
Entity: Ministry of Science and Innovation
Program: Food Science and Food Technology (AGL-ALI)
Official code: AGL2009-13906-C02-01
Start date: 01/01/2010
Finishing date: 30/06/2014
Key words: Nutrition / Polyphenols / Biomarkers / Mediterranean Diet / Metabolomics
SUMMARY
There is a growing and high interest to know the bioavailability of the healthy compounds of food aiming to determine their role in the reduction of the risk to develop illnesses and improvement of the quality of life. The evaluation of the profits/risks ratio of a determined feed and/or of his components requires to know the results obtained in studies that allow to reach conclusions with the maximum scientific evidence on their effects on the organism and from this information to elaborate solid recommendations for the consumers. The main aim of this project is to identify robust, sensitive and reliable biomarkers, based in their bioavailability, activity and relation with the intake of some foods (markers of consumption), and to know their association with the reduction of cardiovascular risk (markers of effect). The study PREDIMED (Prevention with Mediterranean Diet) is a clinical trial of feeding intervention that pretends to evaluate with the maximum level of scientific evidence the efficacy of the Mediterranean diet in the primary prevention of the cardiovascular disease. Until now, 7500 participants with high risk have been included, of which 3874 have been followed during more 3 years. For this study, we will select at random 600 subjects with high risk, divided into the three intervention groups (Mediterranean diet plus olive oil, 200; Mediterranean diet plus nuts, 200) and low-fat diet, 200). All participants will fulfil a food frequency questionnaire, a physical activity questionnaire, and another in which main vascular risk factors will be evaluated. In addition, biological samples will be obtained at baseline, and years 1 and 3.
PUBLICATIONS
- Rotches-Ribalta M; Urpi-Sarda M; Martí MM; Reglero G; Andres-Lacueva C (2013) Resveratrol metabolic fingerprinting after acute and chronic intakes of a functional beverage in humans. Electrophoresis [in press] PubMed
- Boto-Ordoñez M; Rothwell JA; Andres-Lacueva C; Manach C; Scalbert A; Urpi-Sarda M (2013) Prediction of the wine polyphenol metabolic space: An application of the Phenol-Explorer database. Mol Nutr Food Res. [In press] PUBMED
- Boto-Ordoñez M; Urpi-Sarda M; Queipo-Ortuño MI; Corella D; Tinahones FJ; Estruch R; Andres-Lacueva C (2013) Microbial metabolomic fingerprinting in urine after regular dealcoholized red wine consumption in humans Journal of Agricultural and Food Chemistry. 61 (38), pp 9166–9175 PubMed
- Llorach R; Urpi-Sarda M; Tulipani S; Garcia-Aloy, M; Monagas M; Andres-Lacueva C (2013) Metabolomic fingerprint in patients at high risk of cardiovascular disease by cocoa intervention. Molecular Nutrition & Food Research. 2013;57(6):962-973. PubMed
- Zamora-Ros R; Rabassa M; Cherubini A; Urpi-Sarda M; Bandinelli S; Ferrucci L; Andres-Lacueva C (2013) High Concentrations of a Urinary Biomarker of Polyphenol Intake Are Associated with Decreased Mortality in Older Adults. Journal of Nutrition. 143(9):1445-50 PubMed
- Zamora-Ros R; Sefarini M; Estruch R; Lamuela-Raventos RM; Martínez González MA; Salas-Salvadó J; Fiol M; Lapetra J; Arós F; Covas MI; Andres-Lacueva C (2013) Mediterranean diet and non enzymatic antioxidant capacity in the PREDIMED study: Evidence for a mechanism of antioxidant tuning Nutrition, Metabolism and Cardiovascular Diseases. Volume 23, Issue 12, December 2013, Pages 1167–1174. ScienceDirect
- Tulipani S; Llorach R; Urpi-Sarda M; Andres-Lacueva C (2013) Comparative analysis of sample preparation methods to handle the complexity of the blood fluid metabolome: when less is more. Analytical Chemistry. 2013;85(1):341-348. PubMed
- Llorach R; Garcia-Aloy, M; Tulipani S; Vazquez-Fresno R; Andres-Lacueva C (2012) Nutrimetabolomic strategies to develop new biomarkers of intake and health effects. Journal of Agricultural and Food Chemistry. 2012;60(36):8797-8808. PubMed
- Chiva-Blanch G; Ros E; Urpi-Sarda M; Estruch R; Andres-Lacueva C (2012) Reply to Iqbal and Kazory Circulation Research. 2012;111(12):e389-e390
- Rotches-Ribalta M; Andres-Lacueva C; Estruch R; Escribano E; Urpi-Sarda M (2012) Pharmacokinetics of resveratrol metabolic profile in healthy humans after moderate consumption of red wine and grape extract tablets. Pharmacological Research. 2012;66(5):375-82. doi: 10.1016/j.phrs.2012.08.001. PubMed
- Santos-Buelga C; Andres-Lacueva C (2012) Guest editorial - Polyphenols and health (ICPH2011). Archives of Biochemistry and Biophysics. 2012;527(2):65-66. PubMed
- Rotches-Ribalta M; Urpi-Sarda M; Llorach R; Boto-Ordoñez M; Jáuregui O; Chiva-Blanch G; Perez-Garcia L; Jaeger W; Guillén M; Corella D; Tinahones FJ; Estruch R; Andres-Lacueva C (2012) Gut and microbial resveratrol metabolite profiling after moderate long-term consumption of red wine versus dealcoholized red wine in humans by an optimized ultra-high-pressure liquid chromatography tandem mass spectrometry method. Journal of Chromatography A. 2012;1265:105-13. doi: 10.1016/j.chroma.2012.09.093. PubMed
- Tomás-Barberán FA; Andres-Lacueva C (2012) Polyphenols and Health: Current State and Progress Journal of Agricultural and Food Chemistry. 2012;60(36):8773-8775. PubMed
- Vazquez-Fresno R; Llorach R; Alcaro F; Rodríguez MA; Vinaixa M; Chiva-Blanch G; Estruch R; Correig X; Andres-Lacueva C (2012) (1) H-NMR-based metabolomic analysis of the effect of moderate wine consumption on subjects with cardiovascular risk factors. Electrophoresis. 2012;33(15):2345-54. doi: 10.1002/elps.201100646. PubMed
- Zamora-Ros R; Rabassa M; Llorach R; González CA; Andres-Lacueva C (2012) Application of Dietary Phenolic Biomarkers in Epidemiology: Past, Present, and Future. Journal of Agricultural and Food Chemistry. 2012;60(27):6648-6657. PubMed
- Zamora-Ros R; Urpi-Sarda M; Lamuela-Raventos RM; Martínez González MA; Salas-Salvadó J; Arós F; Fitó M; Lapetra J; Estruch R; Andres-Lacueva C (2012) High urinary levels of resveratrol metabolites are associated with a reduction in the prevalence of cardiovascular risk factors in high-risk patients. Pharmacological Research. 2012;65(6):615-620. PubMed
- Tulipani S; Llorach R; Jáuregui O; López-Uriarte, P; Garcia-Aloy, M; Bulló M; Salas-Salvadó J; Andres-Lacueva C (2011) Metabolomics unveils urinary changes in subjects with metabolic syndrome following 12-week nuts consumption. Journal of Proteome Research. 10(11):5047-5058. PubMed
- Zamora-Ros R; Rabassa M; Cherubini A; Urpi-Sarda M; Llorach R; Bandinelli S; Ferrucci L; Andres-Lacueva C (2011) Comparison of 24-h volume and creatinine-corrected total urinary polyphenol as a biomarker of total dietary polyphenols in the InCHIANTI Study Analytica Chimica Acta. 704(1-2):110-115. PubMed
- Urpi-Sarda M; Llorach R; Monagas M; Khan N; Rotches-Ribalta M; Roura E; Lamuela-Raventos RM; Estruch R; Andres-Lacueva C (2011) Effect of cocoa powder in the prevention of cardiovascular disease: biological, consumption and inflammatory biomarkers. A metabolomic approach. En: Muñoz-Torrero D (editor). Recent Advances in Pharmaceutical Sciences. India: Transworld Research Network. pg. 121-132. ISBN: 978-81-7895-528-5.
- Chiva-Blanch G; Urpi-Sarda M; Rotches-Ribalta M; Zamora-Ros R; Llorach R; Lamuela-Raventos RM; Estruch R; Andres-Lacueva C (2011) Determination of resveratrol and piceid in beer matrices by solid-phase extraction and liquid chromatography-tandem mass spectrometry. Journal of Chromatography A. 1218(5):698-705 PubMed
- Llorach R; Garrido I; Monagas M; Urpi-Sarda M; Tulipani S; Bartolome B; Andres-Lacueva C (2010) Metabolomics study of human urinary metabolome modifications after intake of almond (Prunus dulcis (Mill.) D.A. Webb) skin polyphenols. Journal of Proteome Research.9(11):5859-5867 PubMed
- Garrido I; Urpi-Sarda M; Monagas M; Gómez-Cordovés C; Martín-Alvarez PJ; Llorach R; Bartolome B; Andres-Lacueva C (2010) Targeted analysis of conjugated and microbial-derived phenolic metabolites in human urine after consumption of an almond skin phenolic extract. The Journal of Nutrition. 140(10):1799-1807. PubMed
- Bartolome B; Monagas M; Garrido I; Gómez-Cordovés C; Martín-Alvarez PJ; Lebrón-Aguilar R; Urpi-Sarda M; Llorach R; Andres-Lacueva C (2010) Almond (Prunus dulcis (Mill.) D.A. Webb) polyphenols: from chemical characterization to targeted analysis of phenolic metabolites in humans Archives of Biochemistry and Biophysics. 501(1):124-133. PubMed
- Khan N; Monagas M; Llorach R; Urpi-Sarda M; Rabassa M; Estruch R; Andres-Lacueva C (2010) Targeted and metabolomic study of biomarkers of cocoa powder consumption: effects on inflammatory biomarkers in patients at high risk of cardiovascular. Agro Food Industry Hi-Tech. 3(21):51-54.
- Urpi-Sarda M; Ramiro E; Khan N; Ramos-Romero S; Llorach R; Castell M; Gonzalez-Manzano S; Santos-Buelga C; Andres-Lacueva C (2010) Distribution of epicatechin metabolites in lymphoid tissues and testes of young rats with a cocoa-enriched diet. The British Journal of Nutrition. 103(10):1393-1397. PubMed
- Urpi-Sarda M; Llorach R; Khan N; Monagas M; Rotches-Ribalta M; Lamuela-Raventos RM; Estruch R; Tinahones FJ; Andres-Lacueva C (2010) Effect of milk on the urinary excretion of microbial phenolic acids after cocoa powder consumption in humans. Journal of Agricultural and Food Chemistry. 58(8):4706-4711. PubMed
- Llorach R; Urpi-Sarda M; Rotches-Ribalta M; Rabassa M; Andres-Lacueva C (2010) Resveratrol: from dietary intake to promising therapeutic molecule. Agro Food Industry Hi-Tech. 2(21):42-44.
- Llorach R; Jáuregui O; Urpi-Sarda M; Andres-Lacueva C (2010) Methodological aspects for metabolome visualization and characterization: a metabolomic evaluation of the 24 h evolution of human urine after cocoa powder consumption. Journal of Pharmaceutical and Biomedical Analysis. 51(2):373-381. PubMed